March 1, 2024
Biological effects of low-dose γ-ray irradiation on chromosomes and DNA of Drosophila melanogaster

Biological effects of low-dose γ-ray irradiation on chromosomes and DNA of Drosophila melanogaster

Whereas the harm to chromosomes and genes induced by high-dose radiation (HDR) has been nicely researched in lots of organisms, the results of low-dose radiation (LDR), outlined as a radiation dose of ≤100 mSv, are nonetheless being debated. Latest analysis has urged that the organic results of LDR differ from these noticed in HDR. To detect the impact of LDR on genes, we chosen a gene of Drosophila melanogaster, often known as the a number of wing hair (mwh) gene. The hatched heterozygous larvae with genotype mwh/+ have been irradiated by γ-rays of a 60Co supply. After eclosion, the wing hairs of the heterozygous flies have been noticed. The realm of just one or two mwh cells (small spot) and that of greater than three mwh cells (giant spot) have been counted.

The ratio of the 2 sorts of spots have been in contrast between teams irradiated by totally different doses together with a non-irradiated management group. For the small spot in females, the eruption frequency elevated within the teams irradiated with 20-75 mGy, indicating hypersensitivity (HRS) to LDR, whereas within the teams irradiated with 200 and 300 mGy, the frequency decreased, indicating induced radioresistance (IRR), whereas in males, 50 and 100 mGy conferred HRS and 75 and 200 mGy conferred IRR. For the massive spot in females, 75 mGy conferred HRS and 100-800 mGy conferred IRR. In conclusion, HRS and IRR to LDR was present in Drosophila wing cells by delimiting the dose of γ-rays finely, besides within the male giant spot.

Though lead related to intelligence decline in kids has lengthy been reported, research combining intelligence willpower, molecular mechanisms exploration, and biomarker display are fairly uncommon. On this examine, primarily based on 333 kids aged 9-11, we decided the position of DNA methylation (DNAm) within the relationship of lead publicity with kids’s intelligence. DNAm was measured from kids’s blood DNA specimens, and mediation evaluation was carried out to determine DNAm biomarkers mediating the lead-intelligence relationship.

Dynamic Stereoselection of Peptide Helicates and Their Selective Labeling of DNA Replication Foci in Cells

Though largely missed in peptide engineering, coordination chemistry affords a brand new set of interactions that opens unexplored design alternatives for growing complicated molecular buildings. On this context, we report new synthetic peptide ligands that fold into chiral helicates within the presence of labile steel ions comparable to Fe(II) and Co(II). Heterochiral β flip selling sequences encode the stereoselective folding of the peptide ligands and outline the physicochemical properties of their corresponding steel complexes. CD and NMR spectroscopy together with computational strategies allowed us to determine and decide the construction of two isochiral ΛΛ-helicates, folded as topological isomers.
Lastly, along with the in vitro characterization of their selective binding to DNA three-way junctions, cell microscopy experiments demonstrated {that a} rhodamine-labeled Fe(II) helicate was internalized and selectively stains DNA replication factories in practical cells. As well as, blood lead ranges (BLLs) decrease than 100 μg/L nonetheless adversely affected kids’s IQs and DNAm of the 2 fragments. Our knowledge means that DNAm mediates lead-associated cognitive delay in kids and blood lead reference worth for school-aged kids (100 μg/L) ought to be revised, and the candidate biomarkers can be utilized in associated neurological ailments in future.
Biological effects of low-dose γ-ray irradiation on chromosomes and DNA of Drosophila melanogaster
Biological effects of low-dose γ-ray irradiation on chromosomes and DNA of Drosophila melanogaster

Carbon nanotube transmembrane channel formation and single-stranded DNA spontaneous internalization: a dissipative particle dynamics examine

Single-walled carbon nanotube (SWCNT) transmembrane channel formation in a pure 1,2-dimyristoyl-sn-glycero-3-phosphorylcholine (DMPC) bilayer, and the spontaneous internalization of single-stranded DNA (ssDNA) into the shaped pore have been simulated. A mix of computational strategies, Dissipative Particle Dynamics-Monte Carlo hybrid simulations and quantum mechanical calculations on the hybrid-DFT degree, was used as a brand new proposal to carry out DPD simulations granting particular chemical identification to the mannequin particles.
The simulated transmembrane channels confirmed that, within the case of pristine SWCNTs and upon growing the nanotube size, the next tilt angle with respect to the bilayer regular is noticed and extra time is required for the nanotube to stabilize. Alternatively, for SWCNTs with polar rims an virtually perpendicular orientation is most well-liked with lower than 15° of tilt with respect to the bilayer regular as soon as the nanotubes have pierced each monolayers. These findings are supported by experimental observations the place CNTs of common inside diameters of 1.51 ± 0.21 nm and lengths within the 5-15 nm vary have been inserted in DOPC membranes. Furthermore, the narrower the SWCNTs, the slower the spontaneous internalization of ssDNA turns into, and ssDNA ends hydrophobically trapped inside the factitious pore.

Dog Uterus cDNA*

DD-411 30 Reactions
EUR 319

Sheep Uterus cDNA

SD-411 30 reactions
EUR 243

Rabbit Uterus cDNA

TD-411 30 reactions
EUR 243

Bovine Uterus cDNA

BD-411 30 reactions
EUR 243

MiniPig Uterus cDNA

ND-411 30 reactions
EUR 358

Hamster Uterus cDNA

AD-411 30 reactions
EUR 243

Guinea Pig Uterus cDNA

GD-411 30 reactions
EUR 243

Cat Uterus Total RNA*

FR-411 0.05mg
EUR 195

Cat Uterus Genomic DNA

FG-411 0.1mg
EUR 210

Cat Uterus Total Protein

FT-411 1mg
EUR 176

cDNA - Human Tumor Tissue: Uterus

C1235274 40 reactions
EUR 578

Rat Uterus, non-pregnant cDNA

RD-411 30 reactions
EUR 243

Mouse CD1 Uterus, non-pregnant cDNA

MD-411 30 reactions
EUR 243

Mouse BLC Uterus, non-pregnant cDNA

MD-411-BLC 30 reactions
EUR 280

Mouse C57 Uterus, non-pregnant cDNA

MD-411-C57 30 reactions
EUR 280

cDNA - Human Adult Normal Tissue: Uterus

C1234274 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Uterus

C1534274 40 reactions
EUR 578

cDNA - Monkey (Cynomolgus) Normal Tissue: Uterus

C1534274-Cy 40 reactions
EUR 578

cDNA - Human Adult Normal Tissue: Uterus: Cervix

C1234275 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Uterus: Corpus

C1234276-10 10 reactions
EUR 215

cDNA - Human Adult Normal Tissue: Uterus: Fundus

C1234278-10 10 reactions
EUR 215

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Cervix

C1534275 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Corpus

C1534276 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Uterus: Fundus

C1534278 40 reactions
EUR 578

Rat Uterus, non-pregnant cDNA-Random Primer

RD-411-RH 30 reactions
EUR 243

Rat WS Uterus, non-pregnant cDNA-Oligo-dT

RD-411-WS 30 reactions
EUR 243

cDNA - Monkey (Cynomolgus) Normal Tissue: Uterus: Cervix

C1534275-Cy 40 reactions
EUR 578

Mouse C57 Uterus, non-pregnant cDNA-Random Primer

MD-411-C57-RH 30 reactions
EUR 280

Mouse CD1 Uterus, non-pregnant cDNA-Random Primer

MD-411-HR 30 reactions
EUR 243

Dog Uterus RNA*

DR-411 0.05mg
EUR 195

Total RNA - Human Adult Normal Tissue: Uterus: Cervix of uterus

R1234275-50 50 ug
EUR 170

Total RNA - Human Adult Normal Tissue: Uterus: Corpus of Uterus

R1234276-10 10 ug
EUR 191

Fetal Uterus Lysate

XBL-10431 0.1 mg
EUR 632.4
Description: Fetal human uterus tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The fetal human uterus tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the uterus tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The uterus tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

BOVINE, UTERUS, FRESH

8600861 1EA
EUR 67.09

PORCINE, UTERUS, FRESH

8604961 1EA
EUR 65.63

BOVINE, UTERUS, FROZEN

8620861 1EA
EUR 67.09

PORCINE, UTERUS, FROZEN

8624961 1EA
EUR 65.63

OORA00351-10U - UTERUS

OORA00351-10U 1Each
EUR 105
  • UTERUS

OORA00351-1EA - UTERUS

OORA00351-1EA 1Each
EUR 99
  • UTERUS

OORA00493-10U - UTERUS

OORA00493-10U 1Each
EUR 55
  • UTERUS

OORA00493-1EA - UTERUS

OORA00493-1EA 1Each
EUR 59
  • UTERUS

Uterus Membrane Lysate

XBL-11023 0.1 mg
EUR 619.8
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Rat Uterus Fibroblasts

ABC-TC4237 1 vial Ask for price
  • For research use only
Description: Rat uterus fibroblasts, 6-week Wistar rat

Bovine UTERUS 5 EA*

57153-2 5 EA
EUR 245.04

Pig Uterus Total RNA

PR-411 0.1mg
EUR 235

Total RNA - Lupus: Uterus

R1236274Lup-50 50 ug
EUR 421.2

Pig Uterus Genomic DNA

PG-411 0.1mg
EUR 177

Rat Uterus Genomic DNA

RG-411 0.1mg
EUR 177

Sheep Uterus Total RNA

SR-411 0.1mg
EUR 160

Dog Uterus Genomic DNA

DG-411 0.1mg
EUR 210

Rabbit Uterus Total RNA

TR-411 0.1mg
EUR 160

Bovine Uterus Total RNA

BR-411 0.1mg
EUR 160

MiniPig Uterus Total RNA

NR-411 0.1mg
EUR 231

Pig Uterus Total Protein

PT-411 1mg
EUR 153

Sheep Uterus Genomic DNA

SG-411 0.1mg
EUR 177

Human Uterus Genomic DNA

HG-411 0.05mg
EUR 210

Mouse Uterus Genomic DNA

MG-411 0.1mg
EUR 177

Hamster Uterus Total RNA*

AR-411 0.05mg
EUR 160

Dog Uterus Total Protein

DT-411 1mg
EUR 176

Total RNA - Human Adult Normal Tissue 5 Donor Pool: Uterus: Cervix of uterus

R1234275-P 50 ug
EUR 370

Human Uterus Tumor lysate

HTL-1384 1 mg
EUR 927.6

OORA00552-1U - Rat UTERUS

OORA00552-1U 1Each
EUR 60
  • Rat UTERUS

Uterus tumor tissue array

UT802a each
EUR 306
Description: Uterus tumor tissue array, including stromal and leiomyo sarcoma, endometrioid adenocarcinoma, chorionepithelioma, hydatidiform mole, clear cell carcinoma, carcinsarcoma, sarcomatoid carcinoma, with pathology grade, TNM/Stage (AJCC 8th edition), 80 cases/80 cores (cores size 1.5mm), replacing UT802

Rabbit Uterus Genomic DNA

TG-411 0.1mg
EUR 177

Equine Uterus Genomic DNA

GE-411 0.1mg
EUR 210

Bovine Uterus Genomic DNA

BG-411 0.1mg
EUR 177

Uterus Tissue Slide (Normal)

11-401-10um 10 um
EUR 241.8

Uterus Tissue Slide (Normal)

11-401-4um 4 um
EUR 216.6

Uterus Tissue Slide (Benign)

11-402-10um 10 um
EUR 241.8

Uterus Tissue Slide (Benign)

11-402-4um 4 um
EUR 216.6

OORA00552-1EA - Rat UTERUS

OORA00552-1EA 1Each
EUR 59
  • Rat UTERUS

Human Uterus Tissue Lysate

IHUUTETL100UG each
EUR 245
  • Wet Ice
Description: Human Uterus Tissue Lysate

Pig Uterus Frozen Sections

PF-411 10 slides
EUR 261

Sheep Uterus Total Protein

ST-411 1mg
EUR 153

Hamster Uterus Genomic DNA

GA-411 0.1mg
EUR 177

Chicken Uterus Genomic DNA

GC-411 0.1mg
EUR 177

Rabbit UTERUS Y 25 EA*

41253-2 25 EA
EUR 382.07

Rabbit Uterus Total Protein

TT-411 1mg
EUR 153

Bovine Uterus Total Protein

BT-411 1mg
EUR 153

Uterus Tissue Slide (Abnormal)

11-419-10um 10 um
EUR 241.8

Uterus Tissue Slide (Abnormal)

11-419-4um 4 um
EUR 216.6

OORA00523-1U - Rabbit UTERUS

OORA00523-1U 1Each
EUR 255
  • Rabbit UTERUS

Uterus Membrane Tumor Lysate

XBL-11031 0.1 mg
EUR 752.1
Description: Human uterus tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human uterus tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated uterus tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated uterus tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

MiniPig Uterus Total Protein

NT-411 1mg
EUR 176

Pig Uterus Paraffin Sections

PP-411 10 slides
EUR 240

Sheep Uterus Frozen Sections

SF-411 10 slides
EUR 261

Hamster Uterus Total Protein

AT-411 1mg
EUR 153

Chicken Uterus Total Protein

CT-411 1mg
EUR 140

OORA00523-1EA - Rabbit UTERUS

OORA00523-1EA 1Each
EUR 239
  • Rabbit UTERUS

Rabbit Uterus Frozen Sections

TF-411 10 slides
EUR 240

Bovine Uterus Frozen Sections

BF-411 10 slides
EUR 261

Equine Uterus Frozen Sections

EF-411 10 slides
EUR 261

Uterus Tissue Slide (Adenomyoma)

11-432-10um 10 um
EUR 241.8

Uterus Tissue Slide (Adenomyoma)

11-432-4um 4 um
EUR 216.6

OORA00470-10U - Hamster UTERUS

OORA00470-10U 1Each
EUR 75
  • Hamster UTERUS

OORA00470-1EA - Hamster UTERUS

OORA00470-1EA 1Each
EUR 69
  • Hamster UTERUS

MiniPig Uterus Frozen Sections

NF-411 10 slides
EUR 307

Sheep Uterus Paraffin Sections

SP-411 10 slides
EUR 240
A dependence on the nucleotide content material is discovered indicating that the upper the presence of adenine and thymine within the ssDNA chains the slower the internalization turns into, in settlement with the experimental and predicted solvation tendency in water for nucleic acid bases.

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