November 30, 2022
ALKOXYALKYL ESTERS OF NUCLEOTIDE ANALOGS INHIBIT POLYOMAVIRUS DNA REPLICATION AND LARGE T ANTIGEN ACTIVITIES

ALKOXYALKYL ESTERS OF NUCLEOTIDE ANALOGS INHIBIT POLYOMAVIRUS DNA REPLICATION AND LARGE T ANTIGEN ACTIVITIES

Polyomavirus-related infections are ubiqutious in immunocompromised people and in some circumstances are intractable and deadly. On account of lack of accepted medication to deal with polyomavirus infections, cidofovir, a phosphonate nucleotide analog accepted to deal with cytomegalovirus infections has been repurposed as anti-polyomavirus agent. Cidofovir has been modified in varied methods to enhance its efficacies as broad-spectrum antiviral agent.

Nevertheless, the precise mechanisms and targets of cidofovir and its modified derivatives as anti-polyomavirus brokers are nonetheless below analysis. Right here, polyomavirus giant tumor antigens (Tag) actions have been recognized because the viral goal of cidofovir derivatives. The alkoxyalkyl-ester derivatives of cidofovir effectively inhibit polyomavirus DNA replication in cell-free human extracts and a viral in vitro replication system solely using purified proteins. We current proof that DNA helicase, and DNA binding actions of polyomavirus Tags are diminished within the presence of low concentrations of alkoxyalkyl-ester derivatives of cidofovir suggesting that the inhibition of viral DNA replication is at the least partly mediated by inhibiting ssDNA and dsDNA binding actions of Tags.

These findings present that the alkoxyalkyl-ester derivatives of cidofovir are efficient in vitro with out present process additional conversions and conclude that the inhibitory mechanisms of nucleotide analog-based medication are extra complicated than beforehand believed. Research of the ecological results of world change typically give attention to one or just a few species at a time. Consequently, we all know comparatively little in regards to the adjustments underway at real-world scales of organic communities, which usually have a whole bunch or 1000’s of interacting species. Right here, we use COI mtDNA amplicons from month-to-month samples of environmental DNA to survey 221 planktonic taxa alongside a gradient of temperature, salinity, dissolved oxygen and carbonate chemistry in nearshore marine habitat. The result’s a high-resolution image of adjustments in ecological communities utilizing a method replicable throughout all kinds of ecosystems. We estimate community-level variations related to time, area and environmental variables, and use these outcomes to forecast near-term group adjustments on account of warming and ocean acidification.

Environment friendly Gene Suppression by DNA/DNA Double-Stranded Oligonucleotide In Vivo

We just lately reported the antisense properties of a DNA/RNA heteroduplex oligonucleotide consisting of a phosphorothioate DNA-gapmer antisense oligonucleotide (ASO) strand and its complementary phosphodiester RNA/phosphorothioate 2′-O-methyl RNA strand. When α-tocopherol was conjugated with the complementary strand, the heteroduplex oligonucleotide silenced the goal RNA extra effectively in vivo than did the father or mother single-stranded ASO. On this examine, we designed a brand new kind of the heteroduplex oligonucleotide, wherein the RNA portion of the complementary strand was changed with phosphodiester DNA, yielding an ASO/DNA double-stranded construction. The ASO/DNA heteroduplex oligonucleotide confirmed related exercise and liver accumulation as did the unique ASO/RNA design.
Construction-activity relationship research of the complementary DNA confirmed that optimum will increase within the efficiency and the buildup have been seen when the flanks of the phosphodiester DNA complement have been protected utilizing 2′-O-methyl RNA and phosphorothioate modifications. Moreover, analysis of the degradation kinetics of the complementary strands revealed that the DNA-complementary strand in addition to the RNA strand have been fully cleaved in vivo. Our outcomes increase the repertoire of chemical modifications that can be utilized with the heteroduplex oligonucleotide know-how, offering better design flexibility for future therapeutic purposes. Research of the ecological results of world change typically give attention to one or just a few species at a time.
Consequently, we all know comparatively little in regards to the adjustments underway at real-world scales of organic communities, which usually have a whole bunch or 1000’s of interacting species. Right here, we use COI mtDNA amplicons from month-to-month samples of environmental DNA to survey 221 planktonic taxa alongside a gradient of temperature, salinity, dissolved oxygen and carbonate chemistry in nearshore marine habitat. The result’s a high-resolution image of adjustments in ecological communities utilizing a method replicable throughout all kinds of ecosystems. We estimate community-level variations related to time, area and environmental variables, and use these outcomes to forecast near-term group adjustments on account of warming and ocean acidification.
 ALKOXYALKYL ESTERS OF NUCLEOTIDE ANALOGS INHIBIT POLYOMAVIRUS DNA REPLICATION AND LARGE T ANTIGEN ACTIVITIES
ALKOXYALKYL ESTERS OF NUCLEOTIDE ANALOGS INHIBIT POLYOMAVIRUS DNA REPLICATION AND LARGE T ANTIGEN ACTIVITIES

Evaluation of Complicated DNA Rearrangements throughout Early Phases of HAC Formation

Human synthetic chromosomes (HACs) are necessary instruments for epigenetic engineering, for measuring chromosome instability (CIN), and for doable gene remedy. Nevertheless, their use within the latter is probably restricted as a result of the enter HAC-seeding DNA can endure an unpredictable sequence of rearrangements throughout HAC formation. Consequently, after transfection and HAC formation, every cell clone incorporates a HAC with a singular construction that can’t be exactly predicted from the construction of the HAC-seeding DNA. Though it has been reported that these rearrangements can occur, the timing and mechanism of their formation has but to be described.

Rat Klk9(Submandibular glandular kallikrein-9) ELISA Kit

ER0295 96T
EUR 681.12
  • Detection range: 31.25-2000 pg/ml
  • Uniprot ID: P07647
  • Alias: Klk9/klk-9/Tissue kallikrein/Submandibular enzymatic vasoconstrictor(SEV)/KLK-S3/S3 kallikrein
Description: Method of detection: Double Antibody, Sandwich ELISA;Reacts with: Rattus;Sensitivity: 18.75pg/ml

ELISA kit for Rat Submandibular glandular kallikrein-9

EK1729 96 tests
EUR 663.6
Description: Enzyme-linked immunosorbent assay kit for quantification of Rat Submandibular glandular kallikrein-9 in samples from serum, plasma, tissue homogenates and other biological fluids.

Mouse Submandibular gland protein C, Muc19 ELISA KIT

ELI-35955m 96 Tests
EUR 1038

cDNA Synthesis SuperMix

20-abx09801420ulSystems
  • EUR 678.00
  • EUR 577.20
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

Novo? cDNA Kit

M1165-100
EUR 424.8

Novo? cDNA Kit

M1165-25
EUR 320.4

Evo? cDNA Supermix

M1168-100
EUR 457.2

Evo? cDNA Supermix

M1168-25
EUR 320.4

Novo? cDNA Supermix

M1169-100
EUR 529.2

Novo? cDNA Supermix

M1169-25
EUR 346.8

Mouse 16.5 kDa submandibular gland glycoprotein, Spt1 ELISA KIT

ELI-29687m 96 Tests
EUR 1038

Rat Glandular kallikrein- 7, submandibular/renal, Klk7 ELISA KIT

ELI-06139r 96 Tests
EUR 1063.2

cDNA from Plant Normal Tissue: cDNA from Plant: Arabidopsis

C1634310 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Corn

C1634330 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Orange

C1634340 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Potato

C1634350 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Rice

C1634360 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Plant Normal Tissue: cDNA from Plant: Wheat

C1634390 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

First-Strand cDNA Synthesis SuperMix (cDNA up to 12 kb)

20-abx09801620ulSystems
  • EUR 744.00
  • EUR 627.60
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

First-Strand cDNA Synthesis SuperMix (cDNA up to 15 kb)

20-abx09802120ulSystems
  • EUR 1045.20
  • EUR 794.40
  • 100 rxns × 20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

cDNA from Plant Normal Tissue: cDNA from Plant: Soy bean

C1634370 40 reactions
EUR 745.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Epithelial Dissociation System 4 (Epithelial,Submandibular salivary), Mouse and Rat

4-20254 ea Ask for price

Klk9 ELISA Kit| Rat Submandibular glandular kallikrein-9 ELISA K

EF017179 96 Tests
EUR 826.8

Rat Submandibular gland secretory Glx- rich protein CA, Grpca EL

ELI-27901r 96 Tests
EUR 1063.2

Tetro cDNA Synthesis Kit

BIO-65042 30 Reactions Ask for price

Tetro cDNA Synthesis Kit

BIO-65043 100 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053 50 Reactions Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65053/S Sample Ask for price

SensiFAST cDNA Synthesis Kit

BIO-65054 250 Reactions Ask for price

cDNA Probe Diluent Solution

AR0063 5mL
EUR 127.2

cDNA from Arteriosclerosis: Aorta

C1236012Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Artery

C1236013Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Arteriosclerosis: Artery

C1236013Hd-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Vein

C1236020Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Colon

C1236090Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Heart

C1236122Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Heart

C1236122Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Kidney

C1236142Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Kidney

C1236142Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Liver

C1236149Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Asthma: Lung

C1236152Ld-1 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Bronchitis: Lung

C1236152Ld-2 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Emphysema: Lung

C1236152Ld-3 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pneumonia: Lung

C1236152Ld-4 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Lung

C1236152Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Pancreas

C1236188Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spleen

C1236246Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: stomach

C1236248Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Human eNOS cDNA probe

eNOS51-D-2 2 ug
EUR 534

Evo™ cDNA Kit

M1164-100 Ask for price

Evo™ cDNA Kit

M1164-25 Ask for price

Novo? Transcriptome cDNA Kit

M1167-100
EUR 1142.4

Novo? Transcriptome cDNA Kit

M1167-25
EUR 529.2

OneScriptPlus cDNA Synthesis Kit

G235 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis Kit

G236 100 x 20 ul reactions
EUR 202.8

OneScriptPlus cDNA Synthesis SuperMix

G453 25 x 20 ul reactions
EUR 116.4

OneScriptPlus cDNA Synthesis SuperMix

G454 100 x 20 ul reactions
EUR 202.8

circRNA cDNA Synthesis Kit

G627 25 rxn (20 ul/rxn)
EUR 370.8

Plant Tissue cDNA: Arabidopsis

PC34-310 10 rxn
EUR 498

cDNA Synthesis SuperMix for qPCR

20-abx09801920ulSystems
  • EUR 828.00
  • EUR 678.00
  • 100 rxns ×20 ul Systems
  • 50 rxns × 20 ul Systems
  • Shipped within 5-10 working days.

cDNA from Alzheimer's Disease: Brain

C1236035Alz 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Brain

C1236035Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Parkinson's Disease: Brain

C1236035Par 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Dementia: Brain: Hippocampus

C1236052Dem 40 reactions
EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Depression: Brain: Hippocampus

C1236052Dep 40 reactions
EUR 961.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Colon

C1236090Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Esophagus

C1236106Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Heart

C1236122Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from hypertension: Interventricular Septum

C1236130Hd-2 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Kidney

C1236142Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Liver

C1236149Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Lung

C1236152Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Pulmonary Embolism: Lung

C1236152Ld-5 40 reactions
EUR 973.2
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Diaphragm

C1236169Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Pancreas

C1236188Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Skin

C1236218Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Lupus: Spinal Cord

C1236234Lup 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

cDNA from Liver Cirrhosis: Spleen

C1236246Lcs 40 reactions
EUR 801.6
Description: Can be used for various studies in the realm of gene expression, both normal and pathological. It is an excellent control and suitable for educational purposes.

Monkey (Rhesus) cDNA Tissue: Thyroid

MR34-265 10 rxn
EUR 498

Evo? cDNA Kit (gDNA Removal)

M1166-100
EUR 457.2

Human Adult cDNA Tissue: Lung

HA-152 10 rxn
EUR 498

Human Adult cDNA Tissue: Skin

HA-218 10 rxn
EUR 498

Human Adult cDNA Tissue: Testis

HA-260 10 rxn
EUR 498

Human Tumor Tissue: Breast cDNA

HT05-090 10 rxn
EUR 498

Total-Transcriptome cDNA Synthesis Kit

G904 25 reactions
EUR 268.8

Total-Transcriptome cDNA Synthesis Kit

G905 100 reactions
EUR 652.8

Mouse iNOS (macrophage) cDNA probe

iNOS61-D-2 2 ug
EUR 534

Accuris qMax cDNA Synthesis Kit

PR2100-C-100 1 PC
EUR 405.3
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris qMax cDNA Synthesis Kit

PR2100-C-25 1 PC
EUR 170.83
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris qMax cDNA Synthesis Kit

PR2100-C-250 1 PC
EUR 854.12
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

Accuris qMax cDNA Synthesis Kit

PR2100-C-S 1 PC
EUR 93.31
  • To order instruments in 115V / US plug please delete the 'E' off the order code.European 2 pin plugs will be supplied as standard, please request UK if required.

amfiRivert cDNA Synthesis Master Mix

R5101-050 50 rxns
EUR 498

amfiRivert cDNA Synthesis Master Mix

R5101-100 2X50 rxns
EUR 1016.4

amfiRivert cDNA Synthesis Master Mix

R5101-200 4X50 rxns
EUR 1453.2

amfiRivet cDNA Synthesis 2X Buffer

R5102-050 500ul
EUR 160.8
Right here we synthesized a HAC-seeding DNA with two distinct structural domains and launched it into HT1080 cells. We characterised a variety of HAC-containing clones and subclones to trace DNA rearrangements throughout HAC institution. We demonstrated that rearrangements can happen early throughout HAC formation. Subsequently, the established HAC genomic group is stably maintained throughout many cell generations. Thus, early phases in HAC formation seem to at the least often contain a means of DNA shredding and shuffling that resembles chromothripsis, an necessary hallmark of many most cancers sorts. Understanding these occasions throughout HAC formation has crucial implications for future efforts aimed toward synthesizing and exploiting artificial human chromosomes.

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